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Quark Hi, in this video this guy appears to be implying that one single DNA polymerase molecule is responsible for the process of copying DNA. (I may however be reading him wrong.)
He appears to imply that the DNA polymerase simply 'hops' back and forth...
Started by raid517 on
, 4 posts
by 2 people.
Answer Snippets (Read the full thread at scienceforums):
A dimer of some kind requiring the action of two joined (how though?) subunits of DNA polymerase this with transcription and translation )
I think the DNA polymerase III haloenzyme that you mentioned is part, not to be confusing....
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I'm a little confused about this..
What's the difference bw I and III? .I always thought E. coli/bacteria only have DNA poly III...?
Answer Snippets (Read the full thread at studentdoctor):
It and this is what I got
DNA Polymerase III - makes the chain in a 5 to 3 direction and it proofreads with a 3 to 5 exonuclease, so it goes both ways doing something different
DNA Polymerase Polymerase I, II, and III....
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What is the substrate of taq polymerase? Thanks..
Started by Helena on
, 2 posts
by 2 people.
Answer Snippets (Read the full thread at yahoo):
It's the same as other, eukaryotic DNA polymerases (I) - its substrate is deoxyribonucleotides (DNA.
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PCR效果怎么样啊？我们想从买来的基因组中调基因，基因组很有限，所以想用扩增效率优秀的酶.... 0票 票数
Started by 86964109 on
, 4 posts
by 4 people.
Answer Snippets (Read the full thread at dxy):
他们有试用装 可以先试一下 再决定买不买 0票 票数 用过他们的反转录试剂盒，好像感觉比promege的效果好些。 其他的不清楚~ 0票 票数 我用了，挺好的，效率和特异性都不错。比extaq强多了。 0票 票数 .
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Primate Why does RNA have 5 bases and DNA has only 4?
I really don't understand why! RNA is used in the replication of proteins (Because it can fit through the nuclear pores and the DNA cannot) so why would it need to ahve a 5 th base when only 4 are ...
Started by RyanJ on
, 20 posts
by 9 people.
Answer Snippets (Read the full thread at scienceforums):
Specifically:
DNA Bases:
Adenine
Guanine
Cytosine
Thymine
RNA Bases:
Adenine
Guanine
Cytosine
Uracil
So both RNA and DNA employ four bases each, its just that in RNA Uracil takes the....
Is different from the corresponding one in DNA.
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Started by thetripp on
, 12 posts
by 10 people.
Answer Snippets (Read the full thread at reddit):
DNA is the sourcecode; DNA polymerase (DNA->DNA) is the copy/paste function; RNA polymerase (DNA->mRNA) is the runtime; the SpliceosomeI....
It) to say if a particular gene should be expressed or not .
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The term DNA sequencing encompasses biochemical methods for determining the order of the nucleotide bases, adenine, guanine, cytosine, and thymine, in a DNA oligonucleotide. The sequence of DNA constitutes the heritable genetic information in nuclei, ...
Answer Snippets (Read the full thread at orkut):
To each reaction is added only one of the four dideoxynucleotides contaminants affecting... .
Stranded DNA template, a DNA primer, a DNA polymerase, radioactively or fluorescently labeled and dTTP) and the DNA polymerase.
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In welche Richtung bewegt sich nun die DNA-Polymerase III bei der Replikation?
Habe ich das richtig verstanden, dass die DNA-Polymerase den DNA-Einzelstrang jeweils nur in 3'-5' Richtung lesen und Nukleotide hinzufügen kann und dass dann so der Leitstrang...
Started by MartinMartin on
, 4 posts
by 3 people.
Answer Snippets (Read the full thread at chemieonline):
Gruß Vielen Dank für deine klärende Antwort!
Jetzt ist mir aber doch noch eine weitere Frage eingefallen... .
Hi,
die Ableserichtung ist immer 3´-->5´ und Syntheserichtung 5´ --> 3´, daher kann der Folgestrang nicht kontinuirlich synthetisiert werden .
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Hi could anyone please supply a step by step numerical breakdown of the processes involved in DNA replication? I mean I know some processes happen at the same time, but what's the general thrust of things?
It would be really helpful if I could see something...
Started by jebus197 on
, 9 posts
by 2 people.
Answer Snippets (Read the full thread at biology-online):
Video shows RNA-polymerase appearing to 'race along' the DNA molecule and unzip it to create an RNAHi could anyone please supply a step by step numerical breakdown of the processes involved in DNA is in this video here: http://www....
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I have to amplify a 130 bp long single stranded oligo, which we have ordered. Now I have to design primers for amplification. But I don't quite know how to design them. For amplification of double stranded DNA it's easy, but I have troubles for single...
Started by tluebke on
, 7 posts
by 3 people.
Answer Snippets (Read the full thread at biotechniques):
This will then create a full length DNA (with blunt ends if enzyme with proof overhangs in a vector, then only those....
But in the next step the polymerase has no template which can be amplified, because as template (say, 20ng?).
Temperature.
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