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Posted 07 February 2012 - 08:28 AM
Hi,
Odd question - but hear me out!
I'm currently testing the effect of some peptides on the migration of 3T3 fibroblast cells over a scratch wound.
I need to incubate my cells in DMEM media containing the peptide (which...
Started by B.B. on
, 8 posts
by 6 people.
Answer Snippets (Read the full thread at protocol-online):
BUT - Can you freeze-thaw DMEM and it still work optimally?
I can't find anything in DMEM and GM-CSF....
So I would naturally want to freeze my DMEM/Peptagon combination (in aliquots to thaw out when and as needed).
Expensive.
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I wonder if anyone has ever kept records as to how many houses get burned down each year by folks trying to un-thaw pipes?
My favorite is the guy that turns the thermostat off and cranks the wood stove up. I've seen heat pipes freeze and break in the ...
Started by Terry on
, 12 posts
by 8 people.
Answer Snippets (Read the full thread at terrylove):
Anyone ever use the fire....
Please remember to remove all too low.
Just an update on how to thaw frozen pipes and what to do.
I learned this from an old well guy, since now and then .
And fed the hose into the pipe to thaw the ice in the pipe.
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I have come into possession of a giant bag of frozen broccoli-cheddar soup. When it's thawed, I think it will easily fill a 4-quart pot.
What would be the best way to thaw it out? In the fridge, the sink, my slow cooker, or something else?
I live alone...
Started by Inter Alia on
, 11 posts
by 7 people.
Answer Snippets (Read the full thread at straightdope):
Once they're froze....
As with most large things that need in the freezer .
You really should thaw it in the refrigerator.
I would stand it up in a pot at it cross-eyed .
If it's really a gallon, it'll probably take a good 24-48 hours to thaw.
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...I've just realised that we have 2x1,000litre water containers which are frozen solid.
The Great Thaw has started here....and I'm starting to wonder if the sealant around the connectors/taps/etc will cope with the cold ( -16C)and thaw.
The thought of...
Started by Nicos on
, 12 posts
by 10 people.
Answer Snippets (Read the full thread at growfruitandveg):
For the expansion when it freezes, but I'm sure you knew that, just the freeze came upon you sooner than at the top to allow for the expansion when it freezes, but I'm sure you knew that, just the freeze came upon you sooner....
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So I go out the other day to grab a pack of elk from the chest freezer in the
garage and when I open the lid, to my dismay I realize some of the contents have thawed completely or partially. cold air settles to the bottom naturally,
but it was the meat...
Started by greenbuttskunk on
, 13 posts
by 11 people.
Answer Snippets (Read the full thread at ifish):
When you thaw it, just give it the old "sniff test." Meat don't lie about its edibility is you are fine to re-freeze....
Anything thats slacked off and isnt slimy or giving you'll be fine .
Be just fine to refreeze, or to thaw and consume.
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I have a pound of ground pork I wanted to use half of at a time. I accidentally tossed the entire package into the freezer like an idiot without thinking.
While I was considering options, I couldn't help but remember reading somewhere that thawing then...
Started by Leaper on
, 19 posts
by 16 people.
Answer Snippets (Read the full thread at straightdope):
Thaw it, refreeze you thaw the....
I never bothered circumstances? And if so, what are they? I've done this with chicken numerous times .
That you can freeze and thaw meat ONCE while it is raw, and ONCE MORE after it is cooked.
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Hi ladies,
This might sound like a very silly question but I'll ask anyway
I'm hoping to go ahead with a fet April may time depending on how long it takes for this pregnancy to miscarry ( look at my signature ), I'm trying to keep positive and look forward...
Started by Mini Minx on
, 6 posts
by 4 people.
Answer Snippets (Read the full thread at fertilityfriends):
I was lucky in the fact.
I was having 2 put back they said they wud thaw 2 to start and then only get 1 at a time out till me they would thaw each one individually untill they had 2 good embies.
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... am I in trouble?
Coming home from my trucking company's yard in northwest Iowa, a 535 mile trip that was about 80% snow and ice, ... I noticed my washers weren't working.
I was pissed cuz I had just replaced the pump last summer. Eventually the crap...
Started by Tonka on
, 12 posts
by 10 people.
Answer Snippets (Read the full thread at therangerstation):
Really sucks when ya have to spooge yer whiskey in the lines to get where and they freeze up over night...have to....
Some a bottle freeze up.
You are thinking of ethelyne glycol, and that is the toxic stuff in anti-freeze.
Is methyl.
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Interesting article by Harold McGee on quick thawing steaks and other thin cuts. Surprising that there was no negative difference in flavour and texture when steaks were thawed in warm water compared to cold.
http://www.curiouscook.com/site/2011/06/a-...
Started by magste on
, 4 posts
by 3 people.
Answer Snippets (Read the full thread at aussiebbq):
During this time meats is in the actual freezing process....
The only reason to do a slow thaw in the fridge would be to inhibit growth of bacteria - it takes hours for a turkey to thaw at room temperature.
I didn't think there would be.
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Just thawed breast milk for day care tomorrow and I thought it smelled funny. How do you know if its still ok? I froze it on 11-22.
Started by eric and jess on
, 7 posts
by 6 people.
Answer Snippets (Read the full thread at thebump):
I pump more than I use so I freeze whatever's left over at the end of the week, or midweek sometimes.
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