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Posted 01 May 2012 - 10:12 PM
Hi all,
I'm trying to refold my protein (ScFv) from inclusion bodies. First step would be to resolubilize the inclusion bodies with a 8M Urea buffer. However, I have also read somewhere that the Urea buffer needs to have ...
Started by Edmond Ahgumon Chua on
, 1 posts
by 1 people.
Answer Snippets (Read the full thread at protocol-online):
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Posted 30 April 2012 - 12:38 AM
Hi Guys
I'm struggling with one of my protein trying to get soluble proteins. I tried lots of different constructs form that protein that predicted to be structured. I used E coli for over expression diff tags, temp, IPTG...
Started by saad on
, 4 posts
by 2 people.
Answer Snippets (Read the full thread at protocol-online):
You need to defold your protein and the refold it using urea.
If that doesn't work then it gets tricky.
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BLACKFIRE Clay Cleaner & Extender
BLACKFIRE Clay Cleaner & Extender is a revolutionary cleanser that dramatically increases the value you receive from detailing clay by extending its useable life. When the face of detailing clay becomes soiled we used...
Started by Todd@Autopia on
, 4 posts
by 4 people.
Answer Snippets (Read the full thread at camaro5):
I just ordered that new Nanoskin paint prep kit from Detailer's Domain, looking forward to trying.
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This is an interesting history of how paper patterns were born.
Butterick HIstory
The notion that some of the best ideas are developed at the dining room table is certainly true in the pattern world. Can you imagine a world without patterns? Ellen Butterick...
Started by Hettielynn on
, 3 posts
by 3 people.
Answer Snippets (Read the full thread at everythingsewingforum):
Angela Lee.
What a great story.
That's fascinating!
Kathy Wow! Thanks for taking the time to share this .
Thanks for sharing.
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I am taking a personal time-out having gotten angry with DS who just knocked over the just washed laundry while declaring that he was _ not knocking over the laundry. (I said please don't knock the laundry over when I saw it coming--if he were still 3...
Started by Pen on
, 4 posts
by 2 people.
Answer Snippets (Read the full thread at welltrainedmind):
But refold the laundry AFTER he gets some sensory..
Help me pick up and refold the laundry) are perfectly fine, but he needs something to satisfy that need to bang into things.
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CHAPTER 21 BY MREinLa1
Jake and Melissa were up before dawn, gathering supplies and putting the cabin back to rights. Jake took their bag as Melissa did one last walk through to make sure they weren't leaving anything behind. As she stood in the doorway...
Started by ka4ist on
, 1 posts
by 1 people.
Answer Snippets (Read the full thread at radiofreejericho):
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LongR3 IGF-I
Good Science. Great Performance.
The efficient and effective manufacture of recombinant proteins, antibodies, vaccines and viral products in animal cells requires a source of growth factors or some means of growth factor signaling to cells...
Started by JohnnyB on
, 23 posts
by 18 people.
Answer Snippets (Read the full thread at steroidology):
First time trying out tis stuff (did a few cycles of AAS b4 though.
First time trying out tis stuff (did a few cycles of AAS b4 though constituents Long R3 IGF-I 1mg/ml..
Constituents Long R3 IGF-I 1mg/ml..
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I have 2 good rust free doors, 1 has a huge dent and crease in the lower outer skin and the other has a torn out hinge area, but a good skin. Has anybody removed an o.e. skin from one door and installed it on another o.e. door? Is this worth it or should...
Started by chevroletrucks on
, 4 posts
by 3 people.
Answer Snippets (Read the full thread at 67-72chevytrucks):
You are just seeking to bring misery to you and your household by trying to reuse a door.
Great.
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I made two tops using this pattern yesterday, and I have never been so confused trying to follow a pattern. Maybe I was just trying to get them done too quickly, but I found myself trying to put it together with tried and true techniques, only to realize...
Started by niener on
, 15 posts
by 11 people.
Answer Snippets (Read the full thread at everythingsewingforum):
I just couldn't see myself trying to double and roll a narrow hem.
And then you the flounces with the serger.
Twice.
Then you flip that up and begin trying to fit a complete circle to an a-line stay.
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A/N: Back in July, when I got together with the mods, one of our stops was the Queen Mary in Long Beach, CA. She is a sight to behold when you see her for the first time. As we wandered about, there was a photo of thousands of troops heading to WWII on...
Started by PNWgal on
, 14 posts
by 12 people.
Answer Snippets (Read the full thread at moonlightaholics):
And don't knock LaFleur's gator stew till you've tried it, okay.
Mercy.
Again: Have.
For just a little bit, Gibbs shifts from his role as CO to father figure, trying trying.
Is a telling one.
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